Bison code: L.28324
The Netherlands counts 1500 cold cases; 1500 unsolved crimes. This research helps to reduce this number. The aim of the project is to investigate the possibility to obtain a DNA profile from cell material from odour cloths. The project focuses on single cell research, more specifically, isolating single cells and transferring them into a DNA profile to eliminate the mixed profiles that quite often occur. During this research, single cells will be isolated from pieces of evidence coming from actual cold cases! The DNA in these cells will be amplified and detected, which will give a single source DNA-Profile. Cells interact differently with different fabrics, so to find the most efficient way to isolate cells, tests are held on different fibres and structures. Lastly, to determine the probative value of a single source DNA-profile, interviews will take place with different experts in the field.
In addition to the further optimization of the existing processes, a second research line will be explored. Research must be conducted into the possibilities of a lab-on-a-chip application. Some kits have already been chosen for this, but have not yet been tested and compared to each other. Initially, only the amplification yield will be investigated, after that the tests will be repeated with human DNA.